Characterization of Somatic Cells by Determination of Their Volumes with a Coulter Counter
نویسنده
چکیده
To date the usual method for checking variation among populations of somatic cells grown in vitro has been gross microscopic examination . In a previous paper an attempt was made to characterize cell populations in a more quantitative way by measuring cell diameters under the microscope (Simons, 1967) . This kind of characterization can be obtained faster and more exactly when an electronic counter is used to determine the cell volumes. Several authors (Peacock, Williams, and Mengoli, 1960; Brecher et al ., 1962; Richar and Breakell, 1959) have already dealt with size analysis of mammalian cells . It is suggested here that statistical measures be used routinely to characterize cell populations grown in vitro . As a rule, frequency distributions of cell volumes are skew and not normally distributed . It has often been reported (Peacock et al., 1960 ; Brecher et al., 1962; Scherbaum and Rash, 1957) that after logarithmic transformation the cumulative frequency distribution plotted on a probability scale is a straight line, indicating a normal distribution . Therefore, the data obtained by size analysis were fed into an IBM computer and after logarithmic transformation the mean, standard deviation, skewness, and kurtosis were computed for the description of cell volume distributions . These parameters express the shape of near normal frequency distributions quite accurately (Mather, 1951) . In this paper some technical problems of the application of this method are considered . Cell volumes were determined with a Coulter counter Model B (Coulter Electronics, Hialeah, Fla.), connected to a Coulter automatic cell size distribution plotter. For all cell populations, a 70 μ
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 46 شماره
صفحات -
تاریخ انتشار 1970